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Microscopy ..... DF, Rheinberg, Polarization ..... Phase, DIC, Fluorescence ..... Electronic Enhancement

Electronic Enhancement

Video Enhancement

Video enhancementOnce contrast in the microscope image is optimized, the image can be captured and further enhanced using a video camera. These cameras allow integration (amplification) of low light signals and stepwise adjustment of contrast and brightness of the integrated signal. Video contrast enhancement is illustrated in this Entamoeba histolytica imaged by DIC (right).


Increasing Apparent Resolution

Effect of Spatial FilterSome video cameras used for research also have the capability of stepwise adjustment of "sharpness". Much like the sharpen filter in programs like Photoshop ®, this spatial filter enhances the apparent resolution of the image in real time. This is particularly useful in specimens that move quickly - the sharpness can be adjusted once and the enhanced specimen recorded. A Paramecium (right) is seen in both unenhanced and enhanced DIC camera images.


The results of sharpness filtration are particularly evident in the fluorescence image of a bovine pulmonary artery endothelial cell (below) in which the microtubules are so clearly defined. These images were integrated over 1 second. The first image is without any camera-based enhancement. The second image was taken after adjusting the camera's controls for contrast, brightness and sharpness.


Effect of enhancement on fluorescent image




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