Video
Enhancement
Once
contrast in the microscope image is optimized, the image can be captured
and further enhanced using a video camera. These cameras allow
integration (amplification) of low light signals and stepwise adjustment
of contrast and brightness of the integrated signal. Video contrast
enhancement is illustrated in this Entamoeba histolytica imaged by
DIC (right).
Increasing Apparent Resolution
Some
video cameras used for research also have the capability of stepwise
adjustment of "sharpness". Much like the sharpen filter in
programs like Photoshop ®, this spatial filter enhances the apparent
resolution of the image in real time. This is particularly useful in
specimens that move quickly - the sharpness can be adjusted once and
the enhanced specimen recorded. A Paramecium (right) is seen in both
unenhanced and enhanced DIC camera images.
The results of sharpness filtration are particularly evident in the
fluorescence image of a bovine pulmonary artery endothelial cell (below)
in which the microtubules are so clearly defined. These images were integrated
over 1 second. The first image is without any camera-based enhancement.
The second image was taken after adjusting the camera's controls for
contrast, brightness and sharpness.
